Join this interactive session as Felix Schneider discusses his recent paper, “Neuron Replating, a Powerful and Versatile Approach to Study Early Aspects of Neuron Differentiation” with eNeuro Editor-in-Chief Christophe Bernard. Attendees can submit questions at registration and live during the webinar.
Below is the significance statement of Neuron Replating, a Powerful and Versatile Approach to Study Early Aspects of Neuron Differentiation, published on May 6, 2021, in eNeuro and authored by Felix Schneider, Thuy-An Duong, and Marco B. Rust
Unraveling the molecular mechanisms that control neuron differentiation requires reporter gene expression or gene inactivation. In mouse primary hippocampal neurons, a widely used cellular system to study neuron differentiation, classical transfection methods are restricted to later stages of differentiation. Instead, electroporation allows genetic manipulation before seeding. However, time course of reporter gene expression or gene inactivation frequently hinders a full characterization of neuron differentiation, specifically of early stages. To circumvent this limitation, we combined electroporation-based genetic manipulation before initial seeding with a replating step after 2 d in vitro (DIV), which reset neurons into an undifferentiated stage. We show that replated neurons differentiated similar to non-replated neurons. We provide a detailed protocol that allows to comprehensively characterize the molecular mechanisms underlying neuron differentiation.