Module 3B: Generation of Oligodendrocytes from iPS Cells

In this presentation, Hiroko Nobuta will introduce various methods for generating oligodendrocytes from iPS cells. Specifically, Nobuta will:

• Describe various methods of generating mouse and human oligodendrocytes and their pros and cons.
• Introduce the detailed procedure of the growth factor-mediated human oligodendrocyte generation from iPS cells.
• Provide troubleshooting tips for common problems in the growth factor-mediated human oligodendrocyte generation and functional assays.

Learning Objectives

After watching this presentation, participants at all career stages should be able to:

• Describe growth factor-mediated human oligodendrocyte generation from iPS cells.
• Explain how to troubleshoot common experimental challenges.

Supporting Resources

Download this guide to access resources that supplement Nobuta’s presentation.

Speaker

Hiroko Nobuta, PhD

Hiroko Nobuta is a postdoctoral fellow in the neuroscience department at the Albert Einstein College of Medicine. She completed training in oligodendrocyte biology with her PhD mentor James Waschek, at the University of California, Los Angeles, as well as her postdoctoral mentors David Rowitch, at the University of California, San Francisco, and Marius Wernig, at Stanford University. She is now exploring gene engineering methods to manipulate glia cells to treat neurodegenerative disorders.