Material below is adapted from the SfN Short Course Novel Technologies for Single-Cell Resolution Whole-Transcriptome Analysis in CNS Tissue, by Evan Macosko, MD, PhD. Short Courses are day-long scientific trainings on emerging neuroscience topics and research techniques held the day before SfN’s annual meeting.
Scientists do not always agree about how many different types of cells are found in the mammalian brain, nor do they understand all the variation in gene expression that exists even within well-studied categories of cells. But innovations that allow for an efficient look at the whole transcriptomes of tens of thousands of single cells at a time bring the answers to these questions closer than they have ever been before.
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