Material below is adapted from the SfN Short Course Simultaneous Holographic Imaging of Neuronal Circuits in Three Dimensions, by Sean Quirin, PhD, et al. Short Courses are daylong scientific trainings on emerging neuroscience topics and research techniques held the day before SfN’s annual meeting.
Traditional microscopy techniques are limiting in that most only reflect two dimensions of a three-dimensional biological system. Optical imaging, such as two-photon microscopy to measure voltage-sensitive or calcium-sensitive dyes, is minimally invasive and has the resolution to study the individual cells that make up large neuronal networks. However, images from these experiments are captured in two dimensions; to collect data spanning a chunk of tissue, sequential images are scanned one at a time, losing any activity that occurs at the same time in different planes.
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