Material below summarizes the article A New Mouse Line Reporting the Translation of Brain-Derived Neurotrophic Factor Using Green Fluorescent Protein, published on December 27, 2019, in eNeuro and authored by Erin Wosnitzka, Xinsheng Nan, Jeff Nan, Pedro Chacón-Fernández, Lothar Kussmaul, Michael Schuler, Bastian Hengerer, and Yves-Alain Barde.
Highlights
- The direct coupling of the secretory protein brain-derived neurotrophic factor (BDNF) with green fluorescent protein (GFP) interferes with its biosynthesis and secretion.
- Translation can nonetheless be monitored using constructs translating BDNF and GFP as separate proteins.
- To avoid the artefacts resulting from cDNA overexpression, the strategy also involves a substitution of the genomic protein coding sequence, as previously reported with the cytoplasmic protein BRAF.
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About the Contributor
Erin Wosnitzka is completing her PhD Thesis at Cardiff University.
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