Extending the Range of Cellular Imaging: Sodium and Calcium at the Same Time
Material below summarizes the article Simultaneous Sodium and Calcium Imaging from Dendrites and Axons, published on October 14, 2015, in eNeuro and authored by Kenichi Miyazaki and William N. Ross.
Dynamic calcium imaging is an important technique. It can reveal information about the location of various calcium channels and calcium permeable receptors; the time course, magnitude, and location of intracellular calcium concentration ([Ca2+]i) changes; and indirectly, the occurrence of action potentials.
Dynamic sodium imaging, a less-used technique, can reveal analogous information related to sodium signaling. In some cases, like the imaging of AMPA and NMDA receptor responses to synaptic stimulation, detection of both [Ca2+]i and [Na+]i changes in the same preparation may provide more information than separate measurements. This is because both receptors are permeable to sodium, but only the NMDA receptor is permeable to calcium.
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About the Contributors
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