Module 4: Neural Organoids

Learn protocols for generating three-dimensional neural organoid cultures from stem cells and understand applications of these tissues to model brain development and neurological conditions.

Madeline Lancaster
Madeline Lancaster, PhD
Cambridge Biomedical Campus
Aparna Bhaduri
Aparna Bhaduri, PhD
University of California, San Francisco
Madeline Andrews
Madeline Andrews, PhD
University of California, San Francisco

After reviewing Module 4, participants at all career stages should be able to:

  • Describe what the various three-dimensional systems are capable of.
  • Identify examples of human conditions that can be modeled using three-dimensional neural organoid systems.
  • Explain how to use single cell RNA sequencing and neural organoids to approach questions on brain development and disease.
  • Identify resources available to evaluate your own RNA sequencing applications.
Module 4A: Neural Organoids – Modeling Human Brain Development in a Dish
In this presentation, Madeline Lancaster will:

- Introduce development of three-dimensional neural organoids.
- Describe various three-dimensional methods: emphasizing strengths and limitations.
- Provide an overview of neurological conditions so far modeled with organoid systems.
Module 4C: Supporting Resources for Neural Organoids
These resources were selected by Madeline Lancaster, Aparna Bhaduri, and Madeline Andrews, faculty from Module 4: Neural Organoids. These resources supplement their presentations, “Modeling Human Brain Development in a Dish”, and “Using Organoids and Single-Cell RNA Sequencing Approaches to Study Human Cortical Development.”
Module 4B: Using Organoids and Single Cell RNA Sequencing Approaches to Study Human Brain Development
In this presentation, Aparna Bhaduri and Madeline Andrews will:

- Describe human cortical development and relevant cell types.
- Introduce cortical organoid models.
- Introduce single-cell RNA sequencing approaches.
- Provide a summary of findings from organoid studies using RNA sequencing and organoid models.
- Describe scRNAseq methods to better understand cell type.
- Compare organoid and primary cortical cells at a single cell level.
Module 4D: Cell Browser Walkthrough
In this video, Aparna Bhaduri and Madeline Andrews will demonstrate how to use the UCSF Cell Browser.

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