In this presentation, Vikaas Sohal will provide an overview of the “basic operating parameters” for optogenetic tools. Specifically, Sohal will: - Describe how to consider opsin kinetics when designing light trains for stimulation. - Describe how to validate the expression of optogenetic tools. - Identify important considerations when combining optogenetic tools with each other or other optical methods. After watching this presentation, you should be able to understand the critical considerations when selecting optogenetic tools.

Explore resources and articles on chemogenetics.

Explore resources and articles on fos activation.

Explore resources and articles on calcium imaging.

Explore resources and articles on using microscopy techniques for neural circuit interrogation.

A list of the pages contained within each subtopic of the Learning Initiative in Neuroscience: Neural Circuit Interrogation.

LATP webinars cover a wide range of professional development and neuroscience-related topics to help you stay abreast of the latest information and best practices in the field year round.

The following videos are from the 2019 LATP course held by UNAM.