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Born with profound bilateral hearing loss, Tilak Ratnanather is an associate research professor in the Department of Biomedical Engineering at Johns Hopkins University. His research focus is on the analysis of brain structures implicated in neuropsychiatric and neurodevelopmental disorders such as schizophrenia, Alzheimer’s, epilepsy, Huntington’s, deafness and speech, and language processing.
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Poster Training Series TrainingLearn how to produce and characterize human neurons derived from induced pluripotent stem cells (IPSCs). After reviewing Module 2, participants at all career stages should be able to: - Describe techniques and methods used to define human neurons derived from human iPS cells. - Summarize the rationale of directed neuronal differentiation from human pluripotent stem cells. - Describe how to apply the use of small molecules/morphogens to generate major neuron types. - Introduce step-wise quality control criteria to assure the generation of the desired neuron type and troubleshoot the differentiations. - Explain the principles for direct lineage conversion. - Identify the key techniques used to directly generate human neurons from iPS cells by transiently expressing transcription factors. - Describe how to establish long-term culture for disease modeling.






