Dysregulation of Ca2+ homeostasis in cochlear outer hair cells (OHCs) is associated with impaired hearing following noise exposure. Ca2+ signaling in developing OHCs is modulated by oncomodulin (OCM), an EF-hand calcium-binding protein. Here, we investigated whether the lack of OCM disrupts Ca2+ signaling in mature OHCs and influences vulnerability to moderate noise. Using young adult CBA/CaJ mice of either sex, we found that OHCs from Ocm knock-out ( Ocm−/− ) mice showed comparable electromotile responses and synaptic innervation compared with littermate controls. Prior to noise exposure, Ocm −/− mice had auditory brainstem responses with highly variable latencies and amplitudes compared with Ocm +/+ mice. Moderate noise exposure (95 dB SPL, 2 h) caused temporary threshold shifts in wild-type ( Ocm +/+) mice but PTS in Ocm −/− mice. Using a genetically encoded Ca2+ sensor (GCaMP6s) expressed in OHCs, we found increased GCaMP6s fluorescence and ATP-induced Ca2+ signaling in Ocm −/− OHCs. Using GCaMP6s mice...

The capacity of animals to rapidly and appropriately respond to potential threats is critical for survival. In many species, this involves innate defensive behaviors, such as flight or freezing. However, not all threats are dangerous. Habituation allows animals to filter out irrelevant stimuli and avoid unnecessary energy expenditure. While environmental context is known to modulate behavior in associative learning paradigms, it remains unclear whether this also applies to visually evoked defensive behaviors. Here, we address this question in mice of either sex by examining the role of environmental context on habituation of defensive responses to threatening visual stimuli. We developed a protocol that produces rapid (within minutes) and stable (lasting at least one week) habituation of freezing responses to slowly sweeping visual stimuli resembling an aerial predator moving across the sky. Using this protocol, we tested the impact of environmental context on habituation and found that changing the contex...

High-density multielectrode arrays (HD-MEAs) generate large, complex datasets that are challenging to efficiently manage and analyze with existing tools, especially in open-source environments. To address this, we developed the BYU Seizure and Analytics Tool (YSA), an open-source graphical user interface built in Python and C++ for efficient analysis and visualization of HD-MEA recordings. The YSA features raster plots, automated discharge detection and tracking, downsampling, playback, and export functions, enabling streamlined workflows for large-scale neural data. We demonstrate the utility of the tool in the context of seizure and status epilepticus-like activity, highlighting how the YSA facilitates rapid exploration of the spatiotemporal dynamics in brain networks. This platform provides an accessible and practical solution for HD-MEA data analysis, supporting a range of neuroscience applications.

In the article “Different But Complementary Motor Functions Reveal an Asymmetric Recalibration of Upper Limb Bimanual Coordination,” by Ada Kanapskyte, Jesus Alejandro Garcia Arango, Sanjay Joshi, Stephen K. Robinson, Jonathon S. Schofield, Lee M. Miller, Wilsaan M. Joiner, and Weiwei Zhou, which was published …

In this episode, the Editors-in-Chief of the Journal of Neuroscience and eNeuro, Sabine Kastner and Christophe Bernard, discuss the importance of peer review and the progressive review models they have worked to implement at their respective journals. Both agree that, while imperfect, peer review is still a valuable part of the research process. Listen to their conversation to learn how their personal experiences as authors and reviewers shaped their decisions as editors. 

In this webinar, we will demystify MATLAB for new users and brush up on topics for those who are familiar with the program. We will take a step-by-step approach to learn how to develop algorithms, plot graphs for larger data sets, and perform data visualization and analysis.

Huntington’s disease (HD) is a hereditary neurodegenerative disease that typically presents during midlife and is characterized by a combination of motor, cognitive and psychiatric symptoms. HD is fatal and arises from a mutation in the huntingtin ( HTT ) gene, which results in decreased neuronal health followed by brain atrophy, with spiny projection neurons (SPNs) of the striatum being especially vulnerable to degeneration. HTT loss-of-function, caused by haploinsufficiency of the wild-type HTT gene (wt HTT ), is an important feature of HD pathophysiology that has previously been understudied compared to mutant HTT gain-of-function mechanisms. wtHTT is essential for nervous system development and functions as a scaffolding protein to support many vital cellular functions including axonal transport, autophagy and synaptic plasticity. Here, we examined the consequences of wtHTT deletion in the adult cortex and striatum by conditionally inactivating wtHTT in 2–4-month-old male and female Htt fl/fl mice. wtH...

Missense variants in O-GlcNAc transferase (OGT) result in OGT congenital disorder of glycosylation (OGT-CDG), an intellectual disability syndrome associated with O-GlcNAc dyshomeostasis and a range of neurodevelopmental defects. Inhibition of O-GlcNAcase (OGA), the enzyme responsible for removing protein O-GlcNAcylation, has been explored as a target for modulating brain O-GlcNAc homeostasis in neurodegenerative diseases and may also be a target for OGT-CDG. Here, we describe an OGT-CDG mouse line, studied in male mice, that exhibits microcephaly, motor deficits, and brain O-GlcNAc dyshomeostasis, closely mirroring patient symptoms. We genetically explored OGA as a target for OGT-CDG by crossing these mice with a line carrying catalytically inactive OGA. Encouragingly, this partially restored O-GlcNAc homeostasis in brain and blood as determined by Ogt/Oga mRNA ratio. These findings suggest that OGA inhibition can modulate enzymatic imbalance in OGT-CDG mice possessing microcephaly and motor deficits, and ...

Join this interactive session as Yizhou Lyu, Zishan Su, and Yuan Chang Leong discuss their paper, “Hostile Attribution Bias Shapes Neural Synchrony in the Left Ventromedial Prefrontal Cortex during Ambiguous Social Narratives”, with JNeurosci Reviewing Editor Daniela Schiller. Attendees can submit questions at registration and live during the webinar.

Replicability in science, despite being considered a core component of scientific practice, is low across the scientific fields. One possible solution is Registered Reports, an alternative publishing model where peer review is conducted prior to data collection; a study is accepted based on the strength of its hypothesis and methodology, rather than the novelty of the findings or the “story.”